Figure 3.

Wnt/β-catenin signaling pathway in colon tumors. mRNA was isolated from colorectal tumors of AOM/DSS-treated mice, reverse-transcribed to cDNA, and quantitatively assessed with gene-specific primers for (a) adenomatous poliposis coli (Apc), (b) T cell factor 1 (Tcf1), (c) Axin1, (d) β-transducin repeat containing E3 ubiquitin protein ligase pseudogene 1 (bTrcp1), (e) Axin 2, (f) wingless-type MMTV integration site 2b (Wnt2b), (g) glycogen synthase kinase 3β (Gsk3b), (h) Wnt5a, (i) β-catenin, (j) Wnt9a, (k) human homolog of the Drosophila dishevelled gene (Dvl1), and (l) casein kinase I (Ck1). mRNA levels for bTrcp1 in selenium-deficient mice were below limits of detection. Means are presented (N = 4 per bar) with SEM, and were analyzed with two-way ANOVA, followed by Tukey’s post hoc comparisons.