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. 2021 Sep 30;22(19):10651. doi: 10.3390/ijms221910651

Figure 5.

Figure 5

Expression of tight junction Claudin genes. mRNA expression was measured with qPCR in (a,c,e) colon scrapes of control mice and (b,d,f) colon tumors of AOM/DSS-treated mice. Mean (N = 4) + SEM, 2-way ANOVA, followed by Tukey’s post hoc analyses to compare KO vs. WT by diet; letters indicate statistically significant differences. Protein expression of (g) Claudin-1 and Claudin-3, and (h) Claudin-2 in colon scrapes of control mice on selenium-specific diets was assessed with Western blotting, using β-actin as loading control. N = 1–3 per group, 40 µg protein per lane.