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CFPs promote differentiation of T helper 1 and cytotoxic T 1 (Tc1) cells in the mLNs. CFP (50 mg/kg) was intranasally administered to C57BL/6 mice twice at a three days interval. (A) The intracellular levels of TNF-α and IFN-γ in CD4 and CD8 T cells in the mLNs. (B) Mean percentages of IFN-γ-producing (left panel) and TNF-α-producing (right panel) CD4 and CD8 T cells (n = 6, two-way ANOVA, * p < 0.05, ** p < 0.01).
