Figure 1.

SCH772984 significantly attenuates LPS-induced TNFa production and inflammatory genes expression in the RAW 264.7 cell line. (A) IC₅₀ values of TNFa production inhibition for the most effective drugs at 4 h and 24 h post-LPS challenge. (B) Representative Western blot (upper panel) showing the levels of ERK1/2 phosphorylation following SCH772984 treatment upon LPS time course challenge. The graph (lower panel) shows the averaged pERK1/2 abundance normalized to Actb by densitometry analysis of Western blotting bands. Values derived from four independent experiments and their means (+/− SD) are presented. * p ≤ 0.05, as determined by unpaired t-test. (C) qPCR results showing the mRNA expression of inflammatory genes following SCH772984 treatment upon LPS time course. Data from three independent experiments and their means and +/− SD are presented. * p ≤ 0.05, ** p < 0.01, *** p < 0.001 as determined by unpaired t-test.