Figure 8.

CircRNA validation using divergent primers and Sanger sequencing. From the top: the name of the circRNA parent gene, circRNA ID, the amplified band using divergent primers and cDNA, schematic representation of the genomic region of selected circRNAs and their amplified junction, dashed curved arrows represent head-to-tail back-splicing points, the two sets of arrows indicate the position of divergent primers on corresponding exons, conjunction validation using Sanger sequencing, and red arrows show the position of the junction point.