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. 2021 Sep 29;22(19):10550. doi: 10.3390/ijms221910550

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Ultrastructural micrographs using the TEM showing the apoptotic features of HCT 116 cells treated with BCP. HCT 116 cells treated with vehicle (0.5% DMSO) revealed intact cell membrane, normal cytoplasm (CP) with clear nucleus (N), and nucleolus (Ns). In contrast, BCP-treated cells revealed numerous cytoplasmic vacuoles (V), blebbing of the cell membrane (BCM), chromatin condensation (Cc), and disappearance of the nucleolus (Ns), suggesting the features of apoptotic cell death. The pictures were taken after 24 h treatment of vehicle or BCP at 1600× magnification.