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. 2021 Sep 29;22(19):10543. doi: 10.3390/ijms221910543

Figure 2.

Figure 2

Figure 2

Suppression of pro-inflammatory cytokine mRNA expression by geranylgeraniol (GGOH). (a) MG6 cells pre-treated with GGOH at different concentrations for 24 h. Cell viabilities were evaluated using the WST-1 method. (b–e) Pro-inflammatory cytokine mRNA expression levels in MG6 cells pre-treated with GGOH at different concentrations for 24 h followed by LPS (10 ng/mL) treatment for 3 h. (f–h) Pro-inflammatory cytokine mRNA expression levels in MG6 cells pre-treated with GGOH (10 µM) at different times followed by LPS treatment (10 ng/mL) for 3 h. Closed bar, LPS-only treated groups; open bar, GGOH pre-treated groups. The mRNA levels were measured via quantitative reverse transcriptase-PCR, normalized to Eef1a1 (the internal standard), and expressed as fold-changes relative to control cell values or the values corresponding to LPS-only treated MG6 cells. Data are presented as the mean ± S.E.M, n = 3; # p < 0.05 vs. untreated control; * p < 0.05 vs. LPS-only treated group).