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. 2021 Sep 29;22(19):10543. doi: 10.3390/ijms221910543

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of NF-κB p65 nuclear translocation by geranylgeraniol (GGOH) administration. MG6 cells were pre-treated with GGOH (10 µM) for 24 h followed by LPS treatment (10 ng/mL) for 1 h. (a) Cells were fixed and labeled with anti-p65 (red) antibodies. Nuclei were stained with Hoechst 33258 (1 μg/mL) (blue). Scale bars, 20 μm; (b) and (c) Subjection of the cytoplasmic and nuclear fractions of cells fractionated to Western blot analysis. Data are presented as the mean ± S.E.M, n = 3, normalized to total protein levels based on the use of α-tubulin or lamin, and expressed as the fold-change relative to the values corresponding to the control cells. # p < 0.05 vs. untreated control. * p < 0.05 vs. LPS-only treated group).

Inhibition of NF-κB p65 nuclear translocation by geranylgeraniol (GGOH) administration. MG6 cells were pre-treated with GGOH (10 µM) for 24 h followed by LPS treatment (10 ng/mL) for 1 h. (a) Cells were fixed and labeled with anti-p65 (red) antibodies. Nuclei were stained with Hoechst 33258 (1 μg/mL) (blue). Scale bars, 20 μm; (b) and (c) Subjection of the cytoplasmic and nuclear fractions of cells fractionated to Western blot analysis. Data are presented as the mean ± S.E.M, n = 3, normalized to total protein levels based on the use of α-tubulin or lamin, and expressed as the fold-change relative to the values corresponding to the control cells. # p < 0.05 vs. untreated control. * p < 0.05 vs. LPS-only treated group).