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. 2021 Sep 26;22(19):10356. doi: 10.3390/ijms221910356

Figure 10.

Figure 10

The decomposition of B-DNIC-GSH and their conversion into GS-NO induced by heating acidified solutions. (A) the optical absorption spectra recorded 2 and 30 min after acidification of the initial 0.4 mM solution of B-DNIC-GSH (pH 7.4) (curve 1) to pH 1.0 (curves 2 and 3, respectively) and a subsequent 30-s increase in temperature to 80 °C in the air (curve 4). Inset: the weak absorption bands of GS-NO and B-DNIC-GSH at 543 and 768 nm (curves 4 and 1, respectively). (B,C) the optical absorption spectra recorded after acidification of a 0.5 mM solution of B-DNIC-GSH to pH 1.0 (curve 1) and 30-s heating at 80 °C (B) or 25 min incubation at 40 °C (C) in the presence and absence of air (curves 2 and 3, respectively). (D) the absorption spectra of a 9 mM B-DNIC-GSH acidified to pH 1.0 recorded after a 40-fold dilution with distilled water (curve 1) and 15-min incubation at 80 °C in the air followed by a 20-fold dilution of the final solution with distilled water (curve 2) [40]. (With permission).