Figure 11.

Optical absorption spectra of B-DNIC-GSH solutions (5 mM), with molar ratios of “free glutathione” and B-DNIC-GSH of 2: 1 (curve 1) or 1: 1 (curve 2). The solutions were diluted ten times. Curves 3 and 4—the absorption spectra of GS-NO were registered in 5 mM solutions of the same B-DNICs heated at 80 °C with pH = 1.0 in anaerobic conditions for 8–9 min, without being diluted. The spectra are characterized by the absorption band at 334 nm slightly shifted to the short-wave region? And the weak band at 543 nm. Curve 5—the absorption spectrum of the solution, characterizing by curve 4, after diluting that solution four times. The inset shows the spectra registered at higher spectrometer sensitivity in the band absorption region at 543 nm [87]. (With permission).