Figure 20.

The effects of sodium dithionite on the absorption spectra of B-DNIC (0.47 mM as calculated per one iron atom) (pH 7.4) and M-DNIC (0.48 mM) (pH 10.7). Lines 1 and 2—The original absorption spectra of B- and M-DNIC, respectively. Lines 3 and 4—The absorption spectra of B- and M-DNIC treated with sodium dithionite under anaerobic conditions. B-DMIC were obtained by treatment of Fe²⁺ and glutathione solutions with gaseous NO at pH 7.4 at the Fe²⁺: glutathione ratio of 1:10, M-DNIC—with pH increase of the latter solution to pH 10.7 [100]. (With permission).