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Fecal polar gas chromatography−mass spectroscopy (GC−MS). (A) Volcano plot showing fold difference (FD, x-axis) and p-value (y-axis) associated with polar metabolites. (B) Tukey box plots showing the significant abundance of Xyloses, Xylose, and unknown metabolites in MCT2 KO and CO mice. (C) Hierarchical clustering of samples based on the relative abundance of the ASVs yielding the lowest p-values following ANOVA of all ASVs comparing MCT2 KO and CO mice.
