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. 2021 Sep 29;22(19):10510. doi: 10.3390/ijms221910510

Figure 6.

Figure 6

Multiplex fluorescence analysis for enhanced sEV identification and characterization. (A) Purified HT29 sEVs were labeled with anti-CD9-AF488 and anti-CD81-PE either individually (single staining) or in combination (double staining). (B) HT29 sEVs were stained with CTR and anti-CD81-AF488 either individually or in combination. Results are shown as % of labeled particles detected on nFCM, in two fluorescence channels (n = 3; mean ± SEM).