Figure 1.

Chemotherapy pre-treatment modified the lung microenvironment to increase its adhesiveness to cancer cells. (a) A schematic of the lung colonization model with chemotherapeutic agent (+) or vehicle (−) treatment on day 4 before tail vein injection of the indicated breast cancer cells. (b) Left: Quantification of lung cancer cells per field of view (FOV) in mice pre-treated with CTX or vehicle. Right: Same as the left panel except PTX and its vehicle were used. Bottom: Representative images (showing a portion of the FOV). (c) Same as the left panel in (b) except the tGFP-Met1 breast cancer cells were used (n = 6–9 from 2–3 independent experiments). Scale bar: 50 μm. Bars indicate mean ± SEM; Student’s t test; * p < 0.05; ** p < 0.01.