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Active CTX was unable to modify the mLECs to allow more cancer cell adhesion in vitro. (a) A schematic of the in vitro cancer cell-mLEC adhesion assay. (b) Shown is a representative mLEC purity determined by CD31 positivity via flow cytometry. (c) Quantification of tGFP-MVT1 cells per well (in 96-well plates) adhered to mLECs pre-treated with 4-hydroperoxy-cyclophosphamide (4-OOH) (+) or vehicle (−) (n = 6 from 2 independent experiments). Bars indicate mean ± SEM; Student’s t test.
