Figure 5.

CTX increased vascular permeability in the lung. Left: Mice were pre-treated with CTX (+) or vehicle (−), and 4 days later their vascular permeability in the lung was assayed by Evans blue (EB) dye as detailed in Materials and Methods. When indicated, MMPi was injected 24 and 72 h after CTX treatment. After removing intravascular EB by perfusion, EB in the lung parenchyma was extracted by formamide and the nanogram (ng) EB per gram (g) of dried lung was determined as described in Materials and Methods (n = 4–8 from 2 independent experiments). Right: Representative images. Bars indicate mean ± SEM; one-way ANOVA with post hoc Holm–Šídák correction; ** p < 0.01.