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. 2021 Sep 24;22(19):10280. doi: 10.3390/ijms221910280

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Integrin β1 knockdown in cancer cells reduced the effect of CTX. (a) mRNA levels of laminin receptor (LamR) and the indicated integrins (Itg) in tGFP-MVT1 cells were analyzed by reverse transcriptase coupled with quantitative polymerase chain reaction (RT-qPCR). Signals were normalized against glyceraldehyde 3-phosphate dehydrogenase (Gapdh) (from 4 independent experiments). (b) tGFP-MVT1 cells with Itgb1 knockdown (siItgb1) or control knockdown (siControl) were analyzed for their integrin β1 level on the cell surface by flow cytometry to estimate the knockdown efficiency. (c) tGFP-MVT1 cells with siControl or siItgb1 knockdown were injected into mice with CTX (+) or vehicle (−) pre-treatment. Lung cancer cells per FOV at 3 h post-injection were analyzed (n = 6–8 from 2 independent experiments). Bottom: Representative images. Scale bar, 50 μm. Bars indicate mean ± SEM; for panel (c), two-way ANOVA with post hoc Holm–Šídák correction; * p < 0.05.