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. 2021 Sep 26;22(19):10371. doi: 10.3390/ijms221910371

Figure 1.

Figure 1

AK4 is upregulated in primary human pulmonary arterial smooth muscle cells (PASMCs) exposed to hypoxia. (A) Relative mRNA expression of AK1–AK9 genes comprising the adenylate kinase family in PASMCs exposed to 21% O2 (normoxia, NOX) or 1% O2 (hypoxia, HOX) for 72 h; n = 3. (B) Relative mRNA expression of AK4 in PASMCs exposed to normoxia (NOX) or hypoxia (HOX, 1% O2) for the indicated time points, compared to NOX; n = 3. (C) Representative Western blot for AK4 followed by densitometric quantification of relative AK4 expression in PASMCs exposed to normoxia (NOX) or hypoxia (HOX, 1% O2) for the indicated time points, compared to NOX; n = 5. (D) Immunocytochemical localization of AK4 (in green) in PASMCs cultured under normoxia (NOX) or hypoxia (HOX, 1% O2) for 48 h. Nuclear staining with DAPI (in blue). Scale bar: 50 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, (A) unpaired Student’s t-test or (B,C) one-way ANOVA followed by Dunnett multiple comparisons test.