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. 2021 Sep 26;22(19):10371. doi: 10.3390/ijms221910371

Figure 5.

Figure 5

AK4 downregulation decreases the glycolytic metabolism in PASMCs. (A) Representative Western blots for HK2, LDHA, and PDHK1 followed by densitometric quantification of relative expression in PASMCs transfected with siAK4 followed by exposure to normoxia (NOX) or hypoxia (HOX, 1% O2) for 72 h (HK2 and LDHA detection) or 24 h (PDHK1 detection), compared to corresponding control (scrambled siRNA = scramble) under NOX or HOX; n = 4–6. (B) Lactate concentration in PASMC cultured media after AK4 knockdown followed by exposure to normoxia (NOX) or hypoxia (HOX, 1% O2) for 72 h, compared to corresponding control (scramble) under NOX or HOX; n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-way ANOVA followed by Bonferroni multiple comparisons test.