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. 2021 Sep 27;22(19):10397. doi: 10.3390/ijms221910397

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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IL-9 mediated differential gene expression profile during osteoclastogenesis in RA. Cells derived from synovial fluid (SF) of patients with RA were treated with macrophage colony-stimulating factor (M-CSF; 25 ng/mL) and soluble receptor activator of nuclear factor κB ligand (sRANKL; 50 ng/mL) in the presence or absence of rIL-9 (100 ng/mL) for 4 days. After that RNA sequencing was performed with the treated cells as described in Materials and Methods. (A) Volcano plot of differentially expressed genes between M-CSF + sRANKL-treated cells and M-CSF + sRANKL + rIL-9-treated cells. (B) Heatmap of hierarchical cluster analysis of differentially expressed genes between M-CSF + sRANKL-treated cells and M-CSF + sRANKL + rIL-9-treated cells.

IL-9 mediated differential gene expression profile during osteoclastogenesis in RA. Cells derived from synovial fluid (SF) of patients with RA were treated with macrophage colony-stimulating factor (M-CSF; 25 ng/mL) and soluble receptor activator of nuclear factor κB ligand (sRANKL; 50 ng/mL) in the presence or absence of rIL-9 (100 ng/mL) for 4 days. After that RNA sequencing was performed with the treated cells as described in Materials and Methods. (A) Volcano plot of differentially expressed genes between M-CSF + sRANKL-treated cells and M-CSF + sRANKL + rIL-9-treated cells. (B) Heatmap of hierarchical cluster analysis of differentially expressed genes between M-CSF + sRANKL-treated cells and M-CSF + sRANKL + rIL-9-treated cells.