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. 2021 Sep 27;22(19):10397. doi: 10.3390/ijms221910397

Figure 5.

Figure 5

Effect of IL-9 on the expression of matrix metalloproteinases (MMPs). (A–H) Bone explant culture was stimulated with and without rIL-9 (100 ng/mL) for 5 days. Cells were then lysed and homogenized, followed by RNA extraction and cDNA preparation from an equal quantity of RNA. Glyceraldehyde phosphate dehydrogenease (GAPDH) was used as an internal control as the expression of GAPDH showed the least variation with stimulation. Quantitative real-time PCR (RT-PCR) was performed for MMP-1 (A), MMP-2 (B), MMP-3 (C), MMP-8 (D), MMP-9 (E), MMP-12 (F), MMP-13 (G), and MMP-16 (H). The graphs represent the relative expression of these genes (mean  ±  SD; n  =  6). Statistical analysis was performed using paired Student’s t-test comparing treated cells to untreated cells (*: p  ≤  0.05; **: p  ≤  0.005; ns—non significant).