KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal to TRP2 (Dct) | abcam | Cat#ab74073; RRID: AB_1524517 |
| Alexa488-conjugated donkey anti-rabbit IgG | Invitrogen | Cat#R37118; RRID: AB_2556546 |
| Rabbit monoclonal to phosphorylated-S6 (S240/244) | Cell Signaling Technology | Cat#5364; RRID: AB_10694233 |
| Rabbit monoclonal to phosphorylated Akt (Ser473) | Cell Signaling Technology | Cat#4058; RRID: AB_331168 |
| Rabbit monoclonal anti S6 | Cell Signaling Technology | Cat#2217; RRID: AB_331355 |
| Rabbit monoclonal anti Akt | Cell Signaling Technology | Cat#4691; RRID: AB_915783 |
| Rabbit monoclonal anti-GAPDH | Cell Signaling Technology | Cat#2118; RRID:AB_561053 |
| Rabbit polyclonal to TRP2 (Dct) | abcam | Cat#ab74073; RRID: AB_1524517 |
| Alexa488-conjugated donkey anti-rabbit IgG | Invitrogen | Cat#R37118; RRID: AB_2556546 |
| Rabbit monoclonal to phosphorylated-S6 (S240/244) | Cell Signaling Technology | Cat#5364; RRID: AB_10694233 |
| Rabbit monoclonal to phosphorylated Akt (Ser473) | Cell Signaling Technology | Cat#4058; RRID: AB_331168 |
| Rabbit monoclonal anti S6 | Cell Signaling Technology | Cat#2217; RRID: AB_331355 |
| Rabbit monoclonal anti Akt | Cell Signaling Technology | Cat#4691; RRID: AB_915783 |
| Rabbit monoclonal anti-GAPDH | Cell Signaling Technology | Cat#2118; RRID:AB_561053 |
| Rat monoclonal anti-mouse CD4 conjugated with Brilliant Violet 650, clone GK1.5 | Biolegend | Cat#100545; RRID:AB_11126142 |
| Rat monoclonal anti-mouse CD8a conjugated with Alexa Fluor® 488, clone 53-6.7 | Biolegend | Cat#100723; RRID:AB_389304 |
| Hamster monoclonal anti-mouse TCR β chain conjugated with PerCP/Cyanine5.5, cloneH57-597 | Biolegend | Cat#109228; RRID:AB_1575173 |
| Rat monoclonal anti-mouse IL-10 conjugated with PE, clone JES5-16E3 | Biolegend | Cat#505008; RRID:AB_315362 |
| Rat monoclonal anti-mouse TNF-α conjugated with APC, clone MP6-XT22 | Biolegend | Cat# 506308; RRID:AB_315429 |
| Rat monoclonal anti-mouse Ly-6G conjugated with FITC, clone 1A8 | Biolegend | Cat#127606; RRID:AB_1236494 |
| Rat monoclonal anti-mouse CD11b conjugated with APC, clone M1/70 | Biolegend | Cat#101212; RRID:AB_312795 |
| Rat monoclonal anti-mouse CD62L conjugated with APC, clone MEL-14 | Biolegend | Cat#104412; RRID:AB_313099 |
| Rat monoclonal anti-mouse CD45 conjugated with BV510, clone 30-F11 | Biolegend | Cat# 103138; RRID:AB_2563061 |
| Rat monoclonal anti-mouse CD44 conjugated with APC-Cy7, clone IM7 | Biolegend | Cat#103028; RRID:AB_830785 |
| Rat monoclonal anti-mouse CD25 conjugated with Pacific Blue, clone PC61 | Biolegend | Cat# 102022; RRID:AB_493643 |
| Hamster monoclonal anti-mouse CD69 conjugated with Brilliant Violet 711, clone H1.2F3 | Biolegend | Cat# 104537; RRID:AB_2566120 |
| Rat monoclonal anti-mouse F4/80 conjugated with BV510, clone BM8 | Biolegend | Cat#123135; RRID:AB_2562622 |
| Hamster monoclonal anti-mouse CD11c conjugated with Pacific Blue, clone N418 | Biolegend | Cat#117322; RRID:AB_755988 |
| Rat monoclonal anti-mouse MHC Class II (I-AI/E) conjugated with APC-Cy7, clone M5/114.15.2 | Biolegend | Cat#107602; RRID:AB_313317 |
| Mouse monoclonal anti-mouse NK1.1 conjugated with BV650, clone PK136 | Biolegend | Cat#108736; RRID:AB_2563159 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Control diet | Envigo | Teklad-2918 |
| Calcium Alpha-Ketoglutarate Supplemented Diet (2%, 2918) | Envigo | TD.160292 |
| Alpha-ketoglutarate Calcium | Carbosynth | 71686-01-6 |
| Phorbol 12-Myristate 13-Acetate (PMA) | Sigma | Cat#P1585 |
| Ionomycin Calcium Salt | Sigma | Cat#I0634 |
| Brefeldin A | Sigma | Cat# B7651 |
| TRIzol™ Reagent | Invitrogen | Cat#15596026 |
| 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside | Sigma | Cat#B4252 |
| Critical Commercial Assays | ||
| Mouse cytokine/chemokine array 31-Plex | Eve technologies | MD31 |
| Quick-RNA MiniPrep Kit | Zymo | 11-328 |
| High Capacity cDNA RT kit | R&D Systems | Cat#4368813 |
| ProLong Antifade kit | Molecular Probes | Cat#P7481 |
| Foxp3 / Transcription Factor Staining Buffer Set | eBioscience | Cat#00-5523-00 |
| Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye | ThermoFisher | C10337 |
| ISOLATE II RNA mini kit | Bioline | BIO-52073 |
| IL-6 (human) AlphaLISA Detection Kit | Perkin Elmer | AL223C |
| Detergent compatible (DC) protein assay kit | Bio-rad | Cat#5000112 |
| Deposited Data | ||
| Longitudinal Heathspan data (Female and male) | This paper; Mendeley Data | https://doi.org/10.17632/zr4ffhd3k9.1 |
| R Codes for Statistical Data Analysis | This paper; Mendeley Data | https://doi.org/10.17632/ytstmynn4m.1 |
| Experimental Models: Cell Lines | ||
| Human: IMR-90 fetal lung fibroblasts | ATCC | CCL-186 |
| Experimental Models: Organisms/Strains | ||
| Mouse: aged C57BL/6J Female and male mice | Jackson Laboratories | JAX:000664 |
| Oligonucleotides | ||
| See Table S3 | N/A | N/A |
| Software and Algorithms | ||
| OASIS 2 software | Han et al. (2016) Oncotarget 11269 | https://sbi.postech.ac.kr/oasis2/ |
| Prism 8.1.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| R Core team software version 4.0.1 | R Foundation for Statistical Computing | https://cran.r-project.org |
| Python Software | Centrum voor Wiskunde en Informatica | https://www.python.org/downloads/ |
| ImageJ software | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| CalR | Mina et al., 2018 | https://calrapp.org |
| FlowJo 10.6.1 | Becton, Dickinson & Company | https://www.flowjo.com/solutions/flowjo/downloads |
| Other | ||
| Promethion Metabolic Cage System | Sable Systems International | http://www.sablesys.com |
| 31- Clinically relevant frailty phenotype | Susan E. Howlett Whitehead et al., 2014 | https://doi.org/10.1093/gerona/glt136 |
| Expedata-P Data Analysis Software | Sable Systems international | N/A |