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. 2021 Sep 30;22(19):10598. doi: 10.3390/ijms221910598

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of 3 sample preparation procedures in detecting the NAD⁺ metabolome of Hep G2 cells: biphasic extraction (BE), 80% cold methanol (80MeOH) and 40:40:20 with 0.1 M formic acid (40:40:20) extraction (n = 5, average ±SD). Values expressed in ratios of LC-MS/MS signal intensity normalized to internal standard and total protein content (arbitrary units). Nam: nicotinamide; NR: nicotinamide riboside; NMN: nicotinamide mononucleotide; NADP⁺: nicotinamide adenine dinucleotide monophosphate; NADPH: nicotinamide adenine dinucleotide monophosphate, reduced form; NAD⁺: nicotinamide adenine dinucleotide; NADH: nicotinamide adenine dinucleotide, reduced form.