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LC-MS/MS metabolite signal intensity per internal standard for NAD+ and Nam of human whole blood according to: (A), aliquot volume (0.1, 0.5, 1, 2, 4 mL) at blood draw. Average ± SD values from 3 donors (per aliquot and subject n = 1 for 0.1, 0.5 and 2 mL, n = 2 for 4 mL, and n = 3 for 1 mL); (B), number of freeze–thaw cycles (taken on 1, 13, 21 days). Average ± SD values from 3 donors (n = 3). NAD+: nicotinamide adenine dinucleotide; Nam: nicotinamide.
