TALEN	Transcription activator-like effector nuclease
CRISPR-Cas	Clustered regularly interspaced short palindromic repeats-Cas
DSB	Double-strand break
NHEJ	Nonhomologous end joining
HDR	Homology-directed repair
Indel	Nucleotide insertion and deletion
MN	Meganuclease
HE	Homing endonuclease
bp	Base pair
IVC	In vitro compartmentalised system
MT	MegaTAL
TALE	Transcription activator-like effector
RDEB	Recessive dystrophic epidermolysis bullosa
TCRα	T cell receptor alpha
RDEB-K	Primary recessive dystrophic epidermolysis bullosa keratinocyte
RDEB-F	Primary recessive dystrophic epidermolysis bullosa fibroblast
IDLV	Integrase-deficient lentiviral vector
SCID	Severe combined immunodeficiency
XPC	Xeroderma pigmentosum group C
DMD	Duchenne muscular dystrophy
ZFN	Zinc finger nuclease
ZF	Zinc finger
C2H2	Cys2/His2
OPEN	Oligomerised pool engineering
CoDA	Context-dependent assembly
AAV	Adeno-associated virus
LV	Lentiviral vector
HIV	Human immunodeficiency virus
HSPC	Hematopoietic stem and progenitor cell
iPSC	Induced pluripotent stem cell
RVD	Repeat variable diresidue
RVR	Repeat variable residue
SCD	Sickle cell disease
hiPSC	Human induced pluripotent stem cells
AAT	Alpha-1 antitrypsin deficiency
AAVS1	Adeno-associated virus integration site 1
NuFF	Newborn foreskin fibroblast
HPV	Human papillomavirus
bRosa26-EGFP	Bovine rosa26-enhanced green fluorescent protein
RMCE	Recombinase-mediated cassette exchange
HTGTS	High-throughput genome-wide translocation sequencing
Cas	CRISPR-associated protein
crRNA	CRISPR RNA
PAM	Protospacer adjacent motif
tracrRNA	Trans-activating CRISPR RNA
sgRNA	Single guide RNA
dsDNA	Double-stranded DNA
siRNA	Small interfering RNA
2′OMe	2′-O-methyl
2′MOE	2′-O-methoxyethyl
2′F	2′-fluoro
3′PS	3′-phosphorothioate
3′thioPACE	3′thiophosphonoacetate linkage
nCas9	Cas9 nickase
dCas9	Dead Cas9
HTS	High-throughput sequencing
RNP	Ribonucleoprotein
T7E1	T7 endonuclease I
FAST	Far-red light-activated split-Cas9 system
gRNA	Guide RNA
RFN	RNA-guided FokI–dCas9 nuclease
Cas9-pDBD	Programmable DNA-binding domain
hPSC	Human pluripotent stem cell