Figure 1.

Monocrotaline (MCT)-induced pulmonary artery hypertension (PAH) in mice. C57BL/6 mice were treated with or without MCT (right side, 60 mg/kg; IP, once) for 30 days. (A) Hematoxylin and eosin (H&E) staining revealed a thicker pulmonary artery wall (blue arrows) following MCT-induced PAH. A, alveolar; B, bronchial; black arrows, vein. (B) The 3D lung image and vascular tree image were obtained by contrast-enhanced micro-computed tomography (MicroCT). (C,D) The mean pulmonary artery (mPA) and aortic artery (AO) image and PA/AO ratio; (E,F) left ventricle (LV) and right ventricle (RV) image and RV/LV ratio; (G,H) PA area was measured using MicroCT and Image J software. Data are representative of three independent experiments (n = 3), and values are expressed as median and interquartile range. Mann–Whitney U-test was used to compare two independent groups (* p < 0.05).