Figure 4.

Proteomics profiling of HCM-derived exosomes. HCMs were treated with or without MCT for 24 h, and then the exosomal proteins were isolated and analyzed by high-resolution liquid chromatography-mass spectrometry (LC-MS/MS). (A) The intersection of the differentially expressed proteins between the control and MCT-treated samples was subjected to Venn diagram analysis. (B) The fold changes in gene expression between the experimental groups are illustrated with a heatmap. Genes are listed on the y-axis. (C–E) Functional annotation of differentially expressed genes in MCT-treated HCMs were analyzed using gene ontology (GO) barplot, dotplot, and emapplot analyses. (F–H) Extracellular structure organization and regeneration-associated genes identified using these analyses of differentially expressed genes in MCT-treated HCMs. Data are representative of three independent experiments (n = 3).