Figure 6.

DNA adduct formation ex vivo by AAII in rat microsomal fractions of livers (A) and kidneys (B) from control (untreated) and rats pre-treated with AAI, AAII or AAI/AAII mixture, as determined by ³²P-postlabelling. DNA adduct formation ex vivo by AAII in rat cytosolic fractions of livers (C) and kidneys (D) from control (untreated) and rats pre-treated with AAI, AAII or AAI/AAII mixture, as determined by ³²P-postlabelling. All values are given as the means ± SD (n = 3). Numbers above columns (‘F’) indicate fold changes in DNA adduct levels compared to control (untreated). Comparison was performed by t-test analysis: *** p < 0.001, differences from control (untreated). Note: Different scaling was used when comparing AA-DNA adduct levels in microsomal and cytosolic fractions. Negative control incubations were performed either (i) without cytosol or microsomes, (ii) without NADPH, (iii) without DNA or (iv) without AAI and were devoid of DNA adducts.