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. 2021 Sep 25;22(19):10327. doi: 10.3390/ijms221910327

Figure 3.

Figure 3

Different brain regions showed a differential association of 4E-BP2-interacting proteins. Scatter plots of the fluorescence intensity of detected proteins in 4E-BP2 immunoprecipitates in ischemia-reperfusion were represented against control condition, in the cerebral cortex (R3dC vs. SHC3dC) (A) and CA1 region (R3dCA1 vs. SHC3dCA1) (B). Scatter plots of the fluorescence intensity of detected proteins in the CA1 region were also represented against the cerebral cortex in control and IR condition (SHC3dCA1 vs. SHC3dC, and R3dCA1 vs. R3dC) (C,D). Fluorescence intensity was quantified in arbitrary units (A.U.). One dot represents the mean of four experiments. The linear regression fitting line, and Spearman correlation p values were < 0.0001. Heat maps of 49 proteins associated to 4E-BP2 were obtained as fold-changes in IR with respect to their control in both CA1 (ratio R3dCA1/SHC3dCA1) and cortical (ratio R3dC/SHC3dC) regions (E), and in the CA1 region with respect to the cerebral cortex in IR (ratio R3dCA1/R3dC) and control (ratio SHC3dCA1/SHC3dC) conditions (F). Found 4E-BP2-associated proteins were tagged with numbers, and color scale illustrates their fold-change. Differentially associated proteins among the four experimental groups, SHC3dC, SHC3dCA1, R3dC, and R3dCA1, were assessed by ANOVA test (* p < 0.05).