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. 2021 Oct 1;22(19):10678. doi: 10.3390/ijms221910678

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) TLR4, NF-κB (1 and 2), TNFα, and MCP-1 mRNA expression in the hippocampus of SI-AGG and GH mice. Increased levels of TLR4, NF-κB1, TNFα, and MCP1 observed in SI-AGG mice compared with GH or SI-NAG mice (one-way ANOVA: TLR4: * p = 0.03; F = 4.1; TNFα: * p = 0.01; F = 5; MCP-1: * p = 0.01, F = 4.9; NF-κB1: * p = 0.005; F = 2; n= 7–8). No changes in NF-κB2 were found. (B) Representative immunoblot for TLR4, TNFα, pIKKα/β, and NF-κB protein expression. The optical density (OD) of the bands was quantified and normalized relative to the housekeeping protein GAPDH. The bar graphs were obtained using values as means ± S.E.M. of 5–6 samples for the controls (GH) and 6 samples for SI-AGG. The samples were loaded in the same immunoblot, and the values were calculated relative to the samples from GH mice. Individual values are shown in the relative graph plots. For the significance, values were analyzed using unpaired two-tailed t test.

(A) TLR4, NF-κB (1 and 2), TNFα, and MCP-1 mRNA expression in the hippocampus of SI-AGG and GH mice. Increased levels of TLR4, NF-κB1, TNFα, and MCP1 observed in SI-AGG mice compared with GH or SI-NAG mice (one-way ANOVA: TLR4: * p = 0.03; F = 4.1; TNFα: * p = 0.01; F = 5; MCP-1: * p = 0.01, F = 4.9; NF-κB1: * p = 0.005; F = 2; n= 7–8). No changes in NF-κB2 were found. (B) Representative immunoblot for TLR4, TNFα, pIKKα/β, and NF-κB protein expression. The optical density (OD) of the bands was quantified and normalized relative to the housekeeping protein GAPDH. The bar graphs were obtained using values as means ± S.E.M. of 5–6 samples for the controls (GH) and 6 samples for SI-AGG. The samples were loaded in the same immunoblot, and the values were calculated relative to the samples from GH mice. Individual values are shown in the relative graph plots. For the significance, values were analyzed using unpaired two-tailed t test.