Figure 4.

Development of GBOs from: (a) a tumor resected in the operating room coming as a bulk tumor, or (b) already dissociated in small pieces with a surgical blade until reaching less than 500 µm size. (c) Small pieces of tumor are forming GBOs after 15 days in the incubator. (d) GBOs are cut into small fragment pieces and incubated in media at t = 0. (e) GBOs after t = 7 days in culture media and under permanent shaking. (f) GBOs after t = 15 days in culture media and under permanent shaking. GBOs embedded in a matrix to verify invasion properties for (g) 0 h, (h) 24 h, (i) 120 h, and (j) 168 h. Scale bar from (d) to (f): 1000 μm, obtained with x2.5 objective lens, under Evos microscope. Scale bar from (g) to (j): 200 μm, brightfield images were recorded on Cytation 5, using a 4× objective lens.