Figure 8.

The effect of tested compounds (1, 2 and 3) on (a) H₂O₂—induced ROS production, (b) oxaliplatin-induced ROS production (OXA, 25 μM), (c) rotenone-induced ROS production (ROT, 32.5 μM) in SH-SY5Y cells. Intracellular ROS production was determined by DCFH-DA assay as described in experimental procedures. Each point (mean ± SEM of two independent experiments, each of which consisted of eight replicates per treatment group) represents relative fluorescence units and is expressed as a percentage of control compared to corresponding toxin-treated cells (set as 100%). Statistical analysis by one-way ANOVA (GraphPad Prism 8) showed significant differences between the groups (p < 0.05) and was followed by the Dunnett’s multiple comparisons test. Data indicated with *** p ≤ 0.001, * p ≤ 0.05 reflect statistically significant differences between corresponding toxin-treated cells and experimental groups.