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. 2021 Oct 4;22(19):10751. doi: 10.3390/ijms221910751

Figure 1.

Figure 1

Immunohistochemical expression and quantification of IAIPs in the cerebral cortex and Western immunoblot to verify R-22c pAb specificity. Purified human IAIP protein was loaded on lane A. Sheep serum was loaded on lane B. Tissue extraction from the sheep brain was loaded on lane C. The pre-immune serum used as a negative control for the R-22C pAb did not recognize IAIP bands, whereas the R-22c pAb detected two bands, at approximately 250 and 125 kDa in the human IAIP protein, sheep serum, and sheep brain extraction (lane A, B and C).