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. 2021 Oct 7;22(19):10833. doi: 10.3390/ijms221910833

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Schematic of the ablation setup. Green: divergent beam; LS: NIRL laser system; M: silver mirror; TL₁, TL₂: telescope lenses; FL: focusing lens (of 150 mm focal length); SM: two-axis scanning mirror; S: scanning of the sample; MS: manual stage; CE: cooling element (−15 °C); I/O: input/output card; A₁, A₂: analog control signals; PC: computer. GS: glass cover slip; (b,c) Photos of the tissue before and after irradiation; (c) Dashed ellipse: Condensed tissue aerosol. Arrow: indicating an area of lost condensate. Green light: pilot laser (532 nm wavelength); (d) Rendered 3D OCT image of the reference ablations on formalin-fixed murine spleen for ablation volume measurement purposes with the open-source application ParaView 5.9.1 [27]; (e) Manually segmented ablation volumes (with the labels red, blue, green) in the open-source software ITK-Snap 3.8.0 [28].