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. 2021 Oct 7;22(19):10850. doi: 10.3390/ijms221910850

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic analysis of SiICE1 and SiICE2 with orthologous ICE proteins from other plant species, including dicots and monocots. The full-length amino acid sequence alignment of SiICE1, SiICE2, and other ICE proteins was analyzed by using the ClustalW2 program, followed by MEGA X counted 1000 times using the neighbor-joining method [50,51,52]. A phylogenetic tree was then constructed. Except for SiICE1 and SiICE2 of Saussurea involucrata, others include AtICE1 (NP_189309) and AtICE2 (NP_172746) of Arabidopsis thaliana, BnICE1 (AEL33687) of Brassica napus, BrICE1 (ACB70963) of Brassica rapa, CbICE1 (AAS79350) of Capsella bursa-pastoris, CsICE (ACT90640) of Camellia sinensis, EcICE1 (ADY68776) of Eucalyptus camaldulensis, EgICE1 (AEF33833) of Eucalyptus globulus, EsICE (ACT68317) of Eutrema salsugineum, GmICE1 (ACJ39211) of Glycine max, HvICE2 (ABA25896) of Hordeum vulgare, LsICE1 (ADX86750.1) of Lactuca sativa, MdbHLH1 (ABS50251) of Malus domestica, OsICE1(Os11g0523700) and OsICE2 (Os01g0928000) of Oryza sativa, PsICE1 (ABF48720) of Populus suaveolens, PtrICE1 (ABN58427) of Populus trichocarpa, RsICE1 (ADY68771) of Raphanus sativus, TaICE41 (ACB69501) and TaICE87 (ACB69502) of Triticum aestivum, VaICE1 (AGP04217), VaICE2 (AGP04218), and VaICE14 (ADY17816) of Vitis amurensis, VvICE1 (AFI49627), VvICE1a (AGQ03810), and VvICE1b (AGQ03811) of Vitis vinifera, VrICE1 (AGG34704), VrICE2 (AIA58705), VrICE3 (AIA58706), and VrICE4 (AIA58707) of Vitis riparia, and ZmICE2 (ACG46593) of Zea mays.