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. 2021 Oct 7;22(19):10850. doi: 10.3390/ijms221910850

Figure 6.

Figure 6

SiICE1 and SiICE2 differential gene expression in response to cold stress in the callus of snow lotus. Snow lotus callus cultivated for 2 weeks under long-day/light conditions, then dark treatment for 1 week, was divided into two groups. The control group was untreated and cultured at 25 °C, whereas the experimental group was low-temperature treated for 5, 10, or 30 min at 4 °C, respectively. Thereafter, mRNA expression of SiICE1 and SiICE2 was examined, respectively. (A) Phenotype of a snow lotus callus. Scale bar = 1 cm. (B) Semi-qPCR analysis of SiICE1 and SiICE2 gene expression. SiGAPDH represents the reference control of gene expression.