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. 2021 Oct 7;22(19):10850. doi: 10.3390/ijms221910850

Figure 9.

Figure 9

Semi-qRT-PCR of SiICE1 and SiICE2 expression in transgenic Arabidopsis. (A) Seven 35S::SiICE1 transgenic plants, L1, L3, L7, L8, L12, L14, and L15, were randomly selected and semi-qRT-PCR reactions were performed. Wild type (WT) was used as a control group, while Actin2 was employed as a reference control for gene expression at the transcriptional level. (B) Similarly, a semi-qRT-PCR assay was conducted for SiICE2 expression, including L1, L7, L8, L10, L11, L12, L16, and L17 35S::SiICE2 transgenic plants. The lower panel shows the quantitative results of 9A and 9B, respectively. (C,D) Phenotypes of the WT, 35S::SiICE1 (L7, L14), and 35S::SiICE2 (L10, L17) transgenic plants. Seeds for different transgenic Arabidopsis were planted in 1/2 MS agar plates for 2 weeks (shown in (C)). Thereafter, seedlings were cultivated in soil for 14 days, followed by phenotypic analysis and photographed, respectively, as shown in (D). L7 and L14 of 35S::SiICE1 transgenic Arabidopsis and L10 and L17 of 35S::SiICE2 transgenic plants were selected due to relatively higher SiICE1 or SiICE2 gene expression in A and B, respectively.