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. 2021 Oct 7;22(19):10850. doi: 10.3390/ijms221910850

Figure 10.

Figure 10

Transgenic Arabidopsis, 35S::SiICE1 and 35S::SiICE2, cultivated in soil and the effect of cold stress on the phenotype and survival rate are displayed. (A) Seedlings grown on the 1/2 MS media under 22 °C long-day/light conditions for 14 days were transplanted in soil for further cultivation for 7 days under long-day/light conditions. Thereafter, WT, 35S::SiICE1 (L7, L14), or 35S::SiICE2 (L10, L17) transgenic plants were moved to 0 °C for 24 h, then transferred to 22 °C long-day/light conditions for 7 days in order to recover from the cold/freezing stress. Phenotypes were examined and photographed for both the WT and every transgenic plant. (B) Survival rates of the WT, 35S::SiICE1, and 35S::SiICE2 transgenic plants were calculated after cold/freezing treatment. Six independent experiments were performed and 40 seedlings in each group were analyzed by using the Student’s t-test. Asterisks indicate the significant differences in comparison with the control at p < 0.01 (**).