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. 2021 Oct 7;22(19):10850. doi: 10.3390/ijms221910850

Figure 11.

Figure 11

qRT-PCR analysis showing relative mRNA expression of overexpressed 35S::SiICE1 and 35S::SiICE2 transgenic Arabidopsis in response to cold/freezing stress. Seedlings, cultivated under long-day/light conditions for 14 days, of WT, 35S::SiICE1 (L7, L14), or 35S::SiICE2 (L10, L17) transgenic plants were moved to −5 °C for 0 h (room temperature control) or 3 h (low-temperature experimental groups), respectively. Gene-specific primers were employed to perform qPCR reactions in order to detect target gene expression at the mRNA level. Target genes included (A) AtICE1, (B) AtICE2, (C) AtCBF1, (D) AtCBF2, (E) AtCBF3, (F) AtCOR15A, (G) AtCOR47, (H) AtKIN1, and (I) AtRD29A/COR78. Actin2 of Arabidopsis was used as a reference control. Each analyzed value is the average of three experimental replicates and the standard deviation (SD) is calculated for each group. Asterisks indicate significant differences at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).