Figure 3.

Identification of suitable culture supplements for intestinal organoids from the rousette bat. Experimental schema of identifying suitable media components of rousette bat organoids (A). Phase-contrast images of the growth of rousette bat intestinal organoids cultured in base medium alone (Cont) or with different culture supplements. WNR indicates Wnt 3a, Noggin, and R-spondin. Scale bar: 500 µm (B). Cell proliferation ratio of organoids in each culture media (C). The results were shown as a fold increase relative to control and expressed as mean ± S.E.M. * p < 0.05 vs. control.