Skip to main content
. 2021 Oct 5;22(19):10780. doi: 10.3390/ijms221910780

Figure 5.

Figure 5

Silencing of Ctnnb1 gene in mkCF cells. The mkCF cells were transfected with Ctnnb1 siRNA for 72 h and then cultured for 4 further days (7 days in total post-transfection), after which the cells were subjected to molecular and protein analyses. (A) RT-PCR analysis revealed a significant inhibition of Ctnnb1 gene 72 h and 7 days post-transfection compared to control siRNA. (B) Western blot analyses revealed a significant downregulation of β-catenin 7 days post-transfection. ** p < 0.01, **** p < 0.0001 was considered statistically significant. Data represents mean ± SEM of three independent experiments.