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. 2021 Oct 5;22(19):10780. doi: 10.3390/ijms221910780

Figure 6.

Figure 6

Silencing of β-catenin prevents the up-regulation of profibrotic genes in fibroblasts in vitro. mTECs pre-treated with 100 µM AA for 4 h were co-cultured with mkCF or mkCF silenced for the Ctnnb1 gene in the presence or absence of HLSC-EVs. Post-experimental analysis revealed a significant upregulation of the profibrotic genes α-Sma (A) and Col1a1 (B) in fibroblasts exposed to AA-treated mTECs, which was significantly reduced in the presence of HLSC-EVs. No significant up-regulation of both profibrotic genes was observed in fibroblast silenced for the Ctnnb1 gene, which codes for β-catenin in the presence or absence of HLSC-EVs, when compared to control. Data represent the mean ± SD of three independent experiments. A one-way ANOVA with Bonferroni’s comparison test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001 was considered to be statistically significant.