FIG. 2.
The effect of insulin on the subcellular distributions of various proteins in 3T3-L1 adipocytes. 3T3-L1 adipocytes were incubated in the absence (−) or presence (+) of 10−7 M insulin for 20 min at 37°C. Subcellular fractionation was carried out to obtain plasma PM, high density microsome (HDM) and LDM fractions. Immunoblotting was carried out with specific antibodies as indicated. The results shown are from a single preparation of the membrane fractions and are representative of similar determinations.