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Intracellular distribution of GLUT4 in other cell types. The LDM fraction from rat skeletal muscle (A) and an intracellular membrane fraction from CHO cells stably overexpressing GLUT4 (B) were obtained by differential centrifugation and subjected to iodixanol equilibrium gradient centrifugation. The iodixanol fractions were analyzed as described for Fig. 3 by SDS-PAGE followed by immunoblotting with antibodies specific for GLUT4, syntaxin 6 (Syn6) or cellubrevin (Ceb).
