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The experimental analysis of FAM sensors showing the quenching efficiency and measurement of Kd,eff1 and Kd,eff2 values for FenA1-FAM, FenA2-FAM, and FenA5-FAM sensors. (A) FenA1-FAM quenching; (B) FenA1-FAM sensor testing; (C) FenA2-FAM quenching; (D) FenA2-FAM sensor testing; (E) FenA5-FAM quenching; and (F) FenA5-FAM sensor testing. The dabcyl quencher was used in this assay (Figure S4).
