Figure 6.
Evaluation of the binding potential of ssDNA aptamers with ThT. (A) Lighting up with ThT (green line represents FenA2; whereas the red, blue, aqua-blue, the black lines represent FenA1, FenA3, FenA4, and FenA5 ssDNA aptamers). Only FenA2 showed significant improvement in fluorescence upon binding with ThT, and other aptamers had much less affinity for ThT. (B) The FenA2-ThT sensor testing using different concentrations of fenitrothion. The Kd obtained using this method was 33.57 nM (9.307 ppb) and LOD was 14.00 nM (3.881 ppb).