Table 1.
Antibodies | Catalog Number | Host | Dilution | Source | |
---|---|---|---|---|---|
Primary | Cx26, GJB2 | CSB-PA009452LA01HU | Rabbit | 1:50 | Cusabio (Wuhan, China) |
Cx32, GJB1 | CSB-PA008853 | Rabbit | 1:100 | Cusabio (Wuhan, China) | |
Anti-Cx37/GJA4 | ab181701 | Rabbit | 1:300 | Abcam (Cambridge, UK) | |
Anti-Cx40/GJA5 | ab213688 | Rabbit | 1:50 | Abcam (Cambridge, UK) | |
Anti-Cx43/GJA1 | ab87645 | Goat | 1:100 | Abcam (Cambridge, UK) | |
Anti-Cx45/GJA7 | ab135474 | Rabbit | 1:50 | Abcam (Cambridge, UK) | |
Anti-pannexin1/PANX1 | ABN242 | Rabbit | 1:150 | MerckKGaA (Darmstadt, Germany) | |
Human/Mouse/Rat AIF | AF5824 | Sheep | 1:300 | R&DSystems (Minneapolis, MN, SUA) | |
Anti-reelin E-5 | sc-25346 | Mouse | 1:50 | Santa Cruz Bt. (Texas, TX, SUA) | |
P44/42 MAPK (Erk ½) 137F5 | 4695 | Rabbit | 1:250 | Cell Signaling Techn. (Danvers, MA, USA) | |
mTOR Polyclonal | PA5-34663 | Rabbit | 1:100 | Thermo Fisher Scientific (Waltham, MA, SUA) | |
Recombinant Anti-TGF beta1 EPR21143 | ab215715 | Rabbit | 1:100 | Abcam (Cambridge, UK) | |
Secondary | Donkey Anti-Goat IgG Alexa Fluor 488 | ab150129 | Donkey | 1:400 | Abcam (Cambridge, UK) |
Donkey Anti-Rabbit IgG Alexa Fluor 488 | ab150073 | Donkey | 1:400 | Abcam (Cambridge, UK) |
|
Rhodamine (TRITC) AffiniPure Donkey Anti-Rabbit IgG |
711-025-152 | Donkey | 1:400 | Jackson Immuno Research Laboratories, Inc., (Baltimore, PA, SUA) | |
Rhodamine Red–XaffiniPure Donkex Anti-Sheep IgG | 713-295-003 | Donkey | 1:400 | Jackson Immuno Research Laboratories, Inc., (Baltimore, PA, SUA) | |
Anti-mouse IgG Rhodamine Red |
715-295-151 | Donkey | 1:400 | Jackson Immuno Research Laboratories, Inc., (Baltimore, PA, SUA |