Figure 4.

Effects of SPL overexpression and free fatty acids on oxidative stress in rat INS1E insulin-secreting cells. INS1E cells were incubated in the absence or presence of 500 µM PA, OA or a combination of PA and OA (500 µM of each) for 24 h. Thereafter, oxidative stress was estimated by DCFDA oxidation rate. Shown are MEANS ± SEM from n = 6 independent experiments; each condition was measured in triplicate. ANOVA followed by Bonferroni, ** p < 0.01, *** p < 0.001 vs. untreated, # p < 0.05 vs. INS1E-ctr cells treated in the same way.