TABLE 3.
Mutants used in this study
| Mutant | Protein | Protein required fora:
|
|||
|---|---|---|---|---|---|
| 5.8S rRNA processing | mRNA decay | snRNA/snoRNA processing | 5′ ETSb decay | ||
| rrp4-1 | Core exosome component | Yes (21) | Yes (13) | Yes (*) | Yes (9) |
| ski6-100 | Core exosome component | Yes (13) | Yes (13) | Yes (*) | Yes (9) |
| mtr3-1 | Core exosome component | Yes (16) | ND | Yes (*) | Yes (1) |
| rrp6Δ | Nuclear exosome component required for 3′ end processing of 5.8S rRNA | Yes (2) | No (*) | Yes (*) | Yes (1) |
| mtr4-1 | Nuclear DEVH box protein required for 3′ end processing of 5.8S rRNA | Yes (8) | No (*) | Yes (*) | Yes (9) |
| ski2Δ | DEVH box protein required for 3′-to-5′ degradation of mRNA | No (13) | Yes (13) | No (*) | ND |
| ski3Δ | Required for 3′-to-5′ degradation of mRNA | No (13) | Yes (13) | No (*) | ND |
| ski8Δ | Required for 3′-to-5′ degradation of mRNA | No (13) | Yes (13) | No (*) | ND |
a
Reference numbers are given in parentheses. (*), this study; ND, not determined.
b
External transcribed spaces of the pre-rRNA.