Figure 3.

Hela cell cycle analysis with PI: (a): After treatment with 30 µg of Q3G for 24 or 48 h, HELA cells were analyzed for cell cycle distribution with Novocyte flow cytometer. The representative plot shows cells in G₀/G₁ phase (green), S phase (yellow), and G₂/M phase (blue). Compared to normal DMSO treated cells (Control), Q3G treated cells were arrested at S phase and G₂/M phase. (b): Graph shows the percentages of cells in G₀/G, S and G₂/M phase. (c): Data in the table are mean ± SD of two independent experiments in triplicate. * p < 0.05, ** p < 0.01 indicate the significant differences from the control.